Molecular Design Implications

Unlike most of the currently available rFVIII products that are manufactured using a hamster cell line (Chinese Hamster Ovary or Baby Hamster Kidney cells), NUWIQ is produced in human cells.1,2

In fact, NUWIQ is the first and only rFVIII produced in a human cell line without chemical modification or protein fusion.1,2

NUWIQ – Produced in a Human Cell Line

  • Human cells express only human post-translational modifications, like glycosylation and sulfation, to produce a rFVIII protein that closely resembles the FVIII the body produces naturally 3
  • Protein expression in hamster cells results in glycosylation with non-human epitopes, which are potentially antigenic to humans3
  • NUWIQ contains only human glycan epitopes

Glycan Epitopes Found on rFVIII Molecules Produced in Human and Hamster Cells

NUWIQ Contains Only Human Glycan Epitopes

human vs. hampster cell line chart

HEK = Human embryonic kidney; CHO = Chinese hamster ovary; BHK = Baby hamster kidney

NUWIQ – Minimizing Unbound FVIII1,3

  • FVIII and von Willebrand factor (VWF) are naturally bound together in circulation
  • Full sulfation of Tyrosine 1680 (Tyr1680) is vital for VWF-binding affinity and FVIII protection
  • NUWIQ is fully sulfated at Tyr1680, with high VWF-binding affinity and less than 1% unbound FVIII
  • Minimizing unbound FVIII in circulation may help reduce the risk of FVIII inhibitor development 1
nuwiq circles

NUWIQ is Proven Safe & Effective in Adults and Children with Hemophilia A.

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References
  1. Sandberg H, et al. Thromb Res.2012;130:808-817.
  2. Casademunt E, et al. Eur J Haematol. 2012;89:165-176.
  3. Kannicht C, et al. Thromb Res. 2013;131:78-88.